Gro/TLE enables embryonic stem cell differentiation by repressing pluripotent gene expression – University of Copenhagen

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14 November 2014

Gro/TLE enables embryonic stem cell differentiation by repressing pluripotent gene expression

Publication

Adam F. Laing, Sally Lowell, Joshua M. Brickman, Developmental Biology

Embryonic stem cells (ESCs) are karyotypically normal immortal cell lines derived from the mammalian blastocyst. Their two defining characteristics are that they are pluripotent, able to differentiate into all the lineages of the future organism, and they can propagate this potency through cell division, self-renewal.

In this paper the authors have shown that so called Groucho (also know as TLE) proteins are expressed in ESC cultures, particular in cells as they are beginning to differentiate. However, rather than keeping differentiation genes off in stem cells, the authors found that these global co-repressors were essential for turning off stem cell genes as ESCs start to differentiate. The removal of TLE activity through the generation of compound mutants therefore resulted in enhanced self-renewal and a block to differentiation, whereas the overEmbryonic stem cells (ESCs) are karyotypically normal immortal cell lines derived from the mammalian blastocyst. Their two defining characteristics are that they are pluripotent, able to differentiate into all the lineages of the future organism, and they can propagate this potency through cell division, self-renewal.

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You can read the paper HERE


Coauthor: DanStem Professor Joshua Brickman (joshua.brickman@sund.ku.dk)