11 August 2014
Show & Tell - video protocol on growing mini-organs in 3D
Visualization of organogenesis
In close collaboration with researchers at Ecole Polytechnique Fédérale de Lausanne, DanStem researchers have published a video protocol on how to grow mini-pancreas from mouse embryonic progenitor/stem cells in the laboratory. The protocol was published in the scientific, peer-reviewed Journal of Visualized Experiments (JoVE)on July 19, 2014.
In an eight minute long video, the researchers show in a step-by-step manner how to grow mini-pancreas from mouse embryonic progenitor/stem cells under 3D conditions in the laboratory. The video features DanStem Professor Anne Grapin-Botton and her PhD student Manuel Figueiredo-Larsen , who have both worked extensively on refining these 3D methods to provide the best possible conditions for growing mini-pancreatic organs from a few cells.
The pancreas is an essential organ that secretes digestive enzymes and produces the hormones insulin and glucagon which keep our blood glucose level stable. People develop diabetes when the insulin producing cells are damaged or destroyed.
“Our method constitutes an important step forward for diabetes therapy, both in terms of producing mini-organs for drug testing and hopefully for future production of fully functional insulin-producing cells in the laboratory which can replace the beta cells that have been destroyed in the diabetic patients”, says Anne Grapin-Botton.
The methods developed by Anne Grapin-Botton and her colleagues recapitulate many aspects of natural pancreas development. In turn, the knowledge gained from studying how these cells develop under 3D conditions in the laboratory contributes important insights into how cells cooperate to form an organ.
Article details: Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Grapin-Botton, A. In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors, Journal of Visualized Experiments (JoVE),89, e51725, doi:10.3791/51725 (2014). Link: http://www.jove.com/search?q=In+vitro+pancreas+organogenesis.
Contact: Professor Anne Grapin-Botton, firstname.lastname@example.org, +45 2115 2863