08 February 2016
Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors
Rothová, Michaela, Jurriaan J. Hölzenspies, Alessandra Livigni, Santiago Nahuel Villegas & Joshua M. Brickman (2007). Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors. Current Protocols in Stem Cell Biology, 36:1G.3.1-1G.3.12.
Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.