The Serup Laboratory: Developmental biology of the pancreas
Our aim is to understand the developmental biology of the pancreas. We wish to understand the signalling events that regulate growth and differentiation of pancreatic cell types with special emphasis on the insulin producing beta cell.
We are particularly interested in understanding how Notch signalling regulates several distinct processes during pancreatic development. The majority of our studies are done with chicken, mice and embryonic stem cells. We use a wide variety of techniques including gene targeting, transgenic mice, gene arrays, immunohistochemistry, confocal microscopy, tissue explants, and in ovo electroporation. The aim of all our experiments is to understand the genetic and cellular interactions that direct pancreatic organogenesis.
We are currently mapping the temporal requirement of Notch signalling in different processes occurring during pancreatic development as well as determining the cell biological details of Notch signalling events. We are also involved in a collaborative tool-generating project that aims to develop new fluorescent reporters for a number of developmental signalling pathways. These tools will significantly improve our ability to study signalling events in vivo and in vitro.
Horn, Signe, Sune Kobberup, Mette C. Jorgensen, Mark Kalisz, Tino Klein, Ryoichiro Kageyama, Moritz Gegg, Heiko Lickert, Jill Lindner, Mark A. Magnuson, Young-Yun Kong, Palle Serup, Jonas Ahnfelt-Ronne & Jan N. Jensen (2012). Mind bomb 1 is required for pancreatic beta-cell formation. Proceedings of the National Academy of Sciences of the United States of America, 109(19), 7356-7361, doi:10.1073/pnas.1203605109.
Kalisz, Mark, Maria Winzi, Hanne Catherine Bisgaard & Palle Serup (2012). EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression. Developmental Biology, 362(1), 94-103, doi:10.1016/j.ydbio.2011.11.017.
Ahnfelt-Rønne, Jonas, Mette C. Jørgensen, Rasmus Klinck, Jan N. Jensen, Ernst-Martin Füchtbauer, Tye Deering, Raymond J. MacDonald, Chris V.E. Wright, Ole D. Madsen & Palle Serup (2012). Ptf1a-mediated control of Dll1 reveals an alternative to the lateral inhibition mechanism. Development, 139, 39-45, doi: 10.1242/dev.071761.
Ahnfelt-Rønne, Jonas, Philippe Ravassard, Corinne Pardanaud-Glavieux, Raphael Scharfmann & Palle Serup (2010). Mesenchymal Bone Morphogenetic Protein Signaling Is Required for Normal Pancreas Development. Diabetes 59(8), 1948-1956, doi: 10.2337/db09-1010.
Jensen, Jan N., Louise C. Rosenberg, Jacob Hecksher-Sørensen & Palle Serup (2007). Mutant neurogenin-3 in congenital malabsorptive diarrhea. New England Journal of Medicine, 356(17), 1781-1782, doi:10.1056/NEJMc063247.
Pedersen, Jesper K., Shelley B. Nelson, Mette C. Jorgensen, Korinna D. Henseleit, Yoshio Fujitani, Chris V.E. Wright, Maike Sander & Palle Serup (2005). Endodermal expression of Nkx6 genes depends differentially on Pdx1. Developmental Biology, 288(2), 487-501, doi: 10.1016/j.ydbio.2005.10.001.
Heller, R. Scott, Marjorie Jenny, Patrick Collombat, Ahmed Mansouri, Catherine Tomasetto, Ole D. Madsen, Georg Mellitzer, Gerard Gradwohl & Palle Serup (2005). Genetic determinants of pancreatic epsilon-cell development. Developmental Biology 286(1), 217-224, doi: 10.1016/j.ydbio.2005.06.041.
Heller, Scptt R., Doris A. Stoffers, Aihua Liu, Andreas Schedl, E.Bryan Crenshaw III, Ole D. Madsen & Palle Serup (2004). The role of Brn4/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity. Developmental Biology 268(1), 123-134, doi: 10.1016/j.ydbio.2003.12.008.
Held, Jacob, J. Peter Hjorth, Michael S. German, Ole D. Madsen, Palle Serup & Jan Jensen (2003). Activated Notch1 prevents differentiation of pancreatic acinar cells and attenuate endocrine development. Developmental Biology, 260(2), 426-437, doi: 10.1016/S0012-1606(03)00326-9.
Jensen, Jan, Erna Engholm Pedersen, Philip Galante, Jacob Hald, R. Scott Heller, Makoto Ishibashi, Ryoichiro Kageyama, Francois Guillemot, Palle Serup & Ole D. Madsen (2000). Control of endodermal endocrine development by Hes-1. Nature Genetics, 24(1), 36-44, doi:10.1038/71657.