15 May 2020

DanStem PhD course: Introduction to recombinant DNA and NGS techniques and applications

Phd course

The course is designed with early stage researchers in mind and is specifically structured to cover, and help alleviate, many of the common pitfalls when working with recombinant DNA. The topics will be covered in a lecture setting that will include group discussions and workshop style interactions which will draw from participants experiences.

June 24-26, 2020

Registration deadline: June 7, 2020.

Learning objectives

A student who has met the objectives of the course will be able to:

  1. demonstrate a functional understanding of the latest approaches to molecular cloning and recombinant DNA technologies.
  2. apply this knowledge to a set of common experimental tools used in contemporary molecular biology and troubleshoot common experimental pitfalls.
  3. critically evaluate the experimental design of published projects and understand the extent to which they may be prone to artefacts.
  4. use various bioinformatic resources required for effective experimental design and use standard analysis pipelines for processing sequencing data.
  5. choose an appropriate plasmid cloning strategy for the desired purpose and implement it.
  6. design and implement next generation sequencing projects for expression analysis and protein-DNA interaction analysis.

    Content

    The fundamental principles of molecular cloning will be explained with an emphasis on common applications in molecular and cellular biology. The course will build on foundation principals governing how DNA can be manipulated in a laboratory to generate bespoke molecules for a wide range of purposes. Specific applications of these concepts will cover

    1. plasmid cloning, and the generation of transgenic mammalian cell lines using CRISPR/Cas9 technologies

    2. experimental workflows for NGS projects

    3. analytical workflows for NGS projects

    The course is designed with early stage researchers in mind and is specifically structured to cover, and help alleviate, many of the common pitfalls when working with recombinant DNA. The topics will be covered in a lecture setting that will include group discussions and workshop style interactions which will draw from participants experiences.

    The 6 lessons will be followed by a project period of 2 weeks. (equivalent to 20 hours of work), where the participants will design an experiment they wish to pursue for their own research interests, from the topics covered in the lectures, and submit it for evaluation with the course directors. The assignment is done individually, and a satisfactory evaluation is needed to pass the course. During the project period, the participants will have the possibility of individual feedback/discussions with the course directors (by email). The course will be evaluated based on how well the student displayed an understanding of the course material, and how well that was applied to the chosen experimental setting. As this course is intended as an introduction to the topics, there should be clear evidence that students read more in-depth about their chosen experiment approach, and this reading should be referenced in the final proposal. Self-directed research should take up approximately 75% of the allocated project workload (i.e. 15 hours)

    Participants

    Junior PhD students
    Required qualifications: Undergraduate Course in Genetics

    Relevance to graduate programmes

    The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH.

    Language
    English

Enrol online: https://phdcourses.ku.dk/DetailKursus.aspx?id=107478&sitepath=SUND