Anne Grapin-Botton – University of Copenhagen

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Anne Grapin-Botton

Professor of Developmental Biology

anne.grapin-botton@sund.ku.dk

Link to the Grapin-Botton Laboratory Homepage

Research Profile

Anne Grapin-Botton has a background in developmental biology and initially studied nervous system and endoderm development. Her laboratory is currently focusing on pancreas development with the overall goals of understanding how pancreatic cells differentiate during embryogenesis, and determining what limits the pancreatic cells’ regeneration in adults. More specifically, Professor Grapin-Botton and her group investigate the impact of the cellular and organ architecture on the cells’ fate choices and the dynamics of decision processes.

Awards and Honours

Anne Grapin-Botton is the recipient of several awards and grants including, a Human Frontiers Science Program (HFSP) Long Term Fellowship.

Key Recent Discoveries

Lineage tracing uncovers how the founder cells of the pancreas make family trees and we argue that the choices they make to become different cell types are stochastic and depend on their encounter of specific signals
Larsen, H.L., Martín-Coll, L., Nielsen, A.V., Wright, C.V.E., Trusina, A., Kim, Y.H. # and Grapin-Botton, A. # (2017). Stochastic priming and spatial cues orchestrate heterogeneous clonal contribution to mouse pancreas organogenesis. Nature Communications 8, 605, doi:10.1038/s41467-017-00258-4. # Shared last authorship

Molecular profiling of single human stem cells reveals the paths they are taking while differentiating into pancreatic endocrine cells
Petersen, M.B.K., Azad, A., Ingvorsen, C., Hess, K., Hansson, M., Grapin-Botton, A.# and Honoré, C.# (2017). Single-Cell Gene Expression Analysis of a Human ESC Model of Pancreatic Endocrine Development Reveals Different Paths to β-Cell Differentiation. Stem Cell Reports 9, 1246-1261. doi:10.1016/j.stemcr.2017.08.009. * Equal contribution. # Shared last authorship

High resolution movies show how the two daughters of a single pancreatic progenitor become different
Kim, Y. H., List Larsen, H., Rué, P.,  Lemaire, L.A.,  Ferrer, J. and  Grapin-Botton, A. (2015). Cell cycle-dependent differentiation dynamics balances growth and endocrine differentiation in the pancreas. PLOS Biology 13:e1002111, doi:10.1371/journal.pbio.1002111.

Here we establish an in vitro three-dimensional model- organoid- of pancreas development
Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M. and Grapin-Botton, A. (2013). Artificial three-dimensional niches deconstruct pancreas development in vitro. Development 140, 4452-4462, doi: 10.1242/dev.096628.

Here, we show that the three-dimensional architecture of pancreatic cells is important for their differentiation
Cortijo, C., Gouzi, M., Tissir, F. and Grapin-Botton, A. (2012). Planar cell polarity controls pancreatic beta cell differentiation and glucose homeostasis. Cell Reports 2, 1593-1606, doi:10.1016/j.celrep.2012.10.016.

We show that different types of endocrine cells form at different time points during pancreas development
Johansson, K.A., Dursun, U., Jordan, N., Gu, G., Beermann, F., Gradwohl, G., and Grapin-Botton, A. (2007). Temporal Control of Neurogenin3 Activity in Pancreas Progenitors Reveals Competence Windows for the Generation of Different Endocrine Cell Types. Developmental Cell 12, 457-465, doi: 10.1016/j.devcel.2007.02.010.