Flow Cytometry Platform
The Flow Cytometry Platform is a shared resource laboratory operated jointly by the Novo Nordisk Foundation Center for Stem Biology (DanStem) and the NNF Center for Protein Research (CPR). The facility has state-of-the-art equipment and software for cell sorting and analysis, and provides training, support and expertise to researchers at DanStem, CPR, and their affiliates and collaborators in the greater Copenhagen area.
The platform is equipped with three cell sorters and one analyzer. Two of the cell sorters and the analyzer are self-use machines available to trained users. Additionally, the facility provides data acquisition and analysis support with BD FACSDiva, Sony SH800 Sorter Software, ModFit LT, FCS Express and FlowJo. See equipment page.
Several times a year, the platform offers a seminar on the basics of flow cytometry as well as equipment-specific training. The platform also regularly hosts seminars on new and related flow cytometry technology, software and reagents. In addition, the personnel provide support on experimental design, troubleshooting and data analysis.
Publications acknowledging the platform:
Cossarizza, A., Chang, H., Radbruch, A., Acs, A. … (Dela Cruz, G. V.,)… & Zychlinsky, A. (2019), Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49, 1457-1973. doi: 10.1002/eji.201970107.
Lauridsen, Felicia Kathrine Bratt, Tanja Lyholm Jensen, Nicolas Rapin, Derya Aslan, Anna Sofia Wilhelmson, Sachin Pundhir, Matilda Rehn, Franziska Paul, Amir Giladi, Marie Sigurd Hasemann, Palle Serup, Ido Amit & Bo Torben Porse (2018). Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment. Cell Reports 24(3), 766-780, doi: 10.1016/j.celrep.2018.06.057.
M. Löf-Öhlin, Zarah, Pia Nyeng, Matthew E. Bechard, Katja Hess, Eric Bankaitis, Thomas U. Greiner, Jacqueline Ameri, Christopher V. Wright & Henrik Semb (2017) EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity. Nature Cell Biology volume 19, pages 1313–1325, doi: 10.1038/ncb3628
Ameri, Jacqueline, Rehannah Borup, Christy Prawiro, Cyrille Ramond, Karen A. Schachter, Raphael Scharfmann & Henrik Semb (2017). Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors. Cell Reports, 19(1), 36-49, doi:10.1016/j.celrep.2017.03.032.
Anderson, Kathryn G.V., William B. Hamilton, Fabian V. Roske, Ajuna Azad, Teresa E. Knudsen, Maurice A. Canham, Lesley M. Forrester& Joshua M. Brickman (2017). Insulin fine-tunes self-renewal pathways governing naive pluripotency and extra-embryonic endoderm. Nature Cell Biology, 19(10), 1164–1177, doi:10.1038/ncb3617.
Illingworth, Robert S., Jurriaan J. Holzenspies, Fabian V. Roske, Wendy A. Bickmore & Joshua M. Brickman (2016). Polycomb enables primitive endoderm lineage priming in embryonic stem cells. eLife, 5:e14926 doi:10.7554/eLife.14926.
Martin Gonzalez, Javier, Sophie M. Morgani, Robert A. Bone, Kasper Bonderup, Sahar Abelchian, Cord Brakebusch & Joshua M. Brickman (2016). Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports, 7(2), 177-191, doi:10.1016/j.stemcr.2016.07.009.
Hölzenspies, Jurriaan, Gelo Dela Cruz & Brickman Joshua M. (2015). Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture. Methods Mol Biol, doi:10.1007/7651_2015_254.
Morgani, Sophie M. & Josh M. Brickman (2015). LIF supports primitive endoderm expansion during pre-implantation development. Development, doi:10.1242/dev.125021.