Head of Research Platforms

Malte Paulsen

Malte Paulsen is by training a molecular developmental biologist and obtained his PhD with Christof Niehrs at the German Cancer Research Centre and the University of Heidelberg in 2011. After his PhD, Malte switched fields into professional core facility leadership. He started and managed Flow Cytometry core facilities at the Boehringer-Ingelheim funded Institute for Molecular Biology, the Imperial College London, and at the European Molecular Biology Laboratory (EMBL) in Heidelberg.

Malte joined DanStem in November 2020 as head of platforms and laboratory. In his role, Malte is coordinating the activities of the DanStem technology platforms driving tight integration of the platforms activities with the institutes research and adapting them to meet future needs.

The research facilities at DanStem led by Malte Paulsen are known for being active and supportive of joint technology development activities with other leading institutes and R&D prone companies, especially in the field of single-cell resolution readouts. 

Short CV

11/2020, Head of Platforms and Laboratory, DanStem Institute, University Copenhagen, Copenhagen, Denmark

08/2015 - 11/2020 Head of the Flow Cytometry Core Facility, European Molecular Biology, Laboratory Heidelberg (EMBL), Germany

09/2013 - 07/2015 Head of the Cytometry Core Facility at Nation Heart and Lung Institute at the Imperial College London, St. Mary’s Campus, United Kingdom

05/2011 - 08/2013 Head of the Cytometry Core Facility at the Institute of Molecular Biology, Mainz, Germany

07/2006 - 04/2011 University of Heidelberg, PhD program, Dissertation in the Department of Molecular Embryology, DKFZ Heidelberg

List of Publications

Velten, L., Story, B. A., Hernández-Malmierca, P., Raffel, S., Leonce, D. R., Milbank, J., Paulsen, M., Demir, A., Szu-Tu, C., Frömel, R., Lutz, C., Nowak, D., Jann, J. C., Pabst, C., Boch, T., Hofmann, W. H., Müller-Tidow, C., Trumpp, A., Haas, S. and Steinmetz, L. M. (2021). Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics. Nature Communication. doi: 10.1038/s41467-021-21650-1

Velten, L., Story, B. A., Hernandez-Malmierca, P., Milbank, J., Paulsen, M., Lutz, C., Nowak, D., Jann, J. C., Pabst, C., Boch, T., Hofmann, W. K., Mueller-Tidow, C., Raffel, S., Trumpp, A., Haas, S. and Steinmetz, L. M. (2018). MutaSeq reveals the transcriptomic consequences of clonal evolution in acute myeloid leukemia. bioRxiv. doi: 10.1101/500108

Mueller, W. F., Jakob, P., Sun, H., Clauder-Münster, S., Ghidelli-Disse, S., Ordonez, D., Boesche, M., Bantscheff, M., Collier, P., Haase, B., Benes, V., Paulsen, M., Sehr, P., Lewis, J., Drewes, G., and Steinmetz, L. M. (2020). Loss of N-Glycanase 1 Alters Transcriptional and Translational Regulation in K562 Cell Lines. G3: Genes, Genomes, Genetics, 10(5), 1585-1597. doi: 10.1534/g3.119.401031

Gallus, S., Peschke, T., Paulsen, M., Burgahn, T., Niemeyer, C. M., & Rabe, K. S. (2020). Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. ChemBioChem. doi: 10.1002/cbic.202000102

van Jaarsveld, M. T., Deng, D., Ordoñez-Rueda, D., Paulsen, M., Wiemer, E. A., and Zi, Z. (2020). Cell-type-specific role of CHK2 in mediating DNA damage-induced G2 cell cycle arrest. Oncogenesis9(3), 1-7. doi: 10.1038/s41389-020-0219-y

Cossarizza, A., Chang, H., Radbruch, A. … (Paulsen, M.) and Zychlinsky, A. (2019), Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49, 1457-1973. doi: 10.1002/eji.201970107.

Ordoñez‐Rueda, D., Baying, B., Pavlinic, D., Alessandri, L., Yeboah, Y., Landry, J. J., Calogero, R., Benes , V., and Paulsen, M. (2020). Apoptotic Cell Exclusion and Bias‐Free Single‐Cell Selection Are Important Quality Control Requirements for Successful Single‐Cell Sequencing Applications. Cytometry Part A97, 156-167. doi: 10.1002/cyto.a.23898

Tomlin, F. M., Gerling-Driessen, U. I., Liu, Y. C., Flynn, R. A., Vangala, J. R., Lentz, C. S., Clauder-Muenster, S., Jakob, P., Mueller, W. F., Ordoñez-Rueda, D., Paulsen, M., Matsui, N., Foley, D., Rafalko, A., Suzuki, T., Bogyo, M., Steinmetz, L. M., Radhakrishnan, S. K., and Bertozzi, C. R. (2017). Inhibition of NGLY1 inactivates the transcription factor Nrf1 and potentiates proteasome inhibitor cytotoxicity. ACS central science3, 1143-1155. doi: 10.1021/acscentsci.7b00224

Dhariwal, J., Cameron, A., Trujillo-Torralbo, M. B., Del Rosario, A., Bakhsoliani, E., Paulsen, M., Jackson, D. J., Edwards, M. R., Rana, B. M. J., Cousins, D.J.,  2,5,6, Hansel, T.,  Johnston, S. L., and Walton, R. P. (2017). Mucosal type 2 innate lymphoid cells are a key component of the allergic response to aeroallergens. American journal of respiratory and critical care medicine195(12), 1586-1596. doi: 10.1164/rccm.201609-1846OC

Bernardo, D., Durant, L., Mann, E. R. … (Paulsen, M.) and Knight, S. C. (2016). Chemokine (CC motif) receptor 2 mediates dendritic cell recruitment to the human colon but is not responsible for differences observed in dendritic cell subsets, phenotype, and function between the proximal and distal colon. Cellular and molecular gastroenterology and hepatology2, 22-39. doi: 10.1016/j.jcmgh.2015.08.006

Zeino, M., Paulsen, M. S., Zehl, M., Urban, E., Kopp, B., and Efferth, T. (2015). Identification of new P-glycoprotein inhibitors derived from cardiotonic steroids. Biochemical pharmacology93, 11-24. doi: 10.1016/j.bcp.2014.10.009

Seo, E. J., Wiench, B., Hamm, R., Paulsen, M., Zu, Y., Fu, Y., and Efferth, T. (2015). Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres. Phytomedicine22, 438-443. doi: 10.1016/j.phymed.2015.01.012

Wiench, B., Chen, Y. R., Paulsen, M., Hamm, R., Schröder, S., Yang, N. S., and Efferth, T. (2013). Integration of different “-omics” technologies identifies inhibition of the IGF1R-Akt-mTOR signaling cascade involved in the cytotoxic effect of shikonin against leukemia cells. Evidence-Based Complementary and Alternative Medicine2013, 818709–818711. doi: 10.1155/2013/818709

Wiench, B., Eichhorn, T., Korn, B., Paulsen, M., and Efferth, T. (2012). Utilizing inherent fluorescence of therapeutics to analyze real-time uptake and multi-parametric effector kinetics. Methods57, 376-382. doi: 10.1016/j.ymeth.2012.01.007

Wiench, B., Eichhorn, T., Paulsen, M., and Efferth, T. (2013). Shikonin directly targets mitochondria and causes mitochondrial dysfunction in cancer cells. Evidence-Based Complementary and Alternative Medicine2012. doi: 10.1155/2012/726025

Paulsen, M., Legewie, S., Eils, R., Karaulanov, E., and Niehrs, C. (2011). Negative feedback in the bone morphogenetic protein 4 (BMP4) synexpression group governs its dynamic signaling range and canalizes development. Proceedings of the National Academy of Sciences108, 10202-10207. doi: 10.1073/pnas.1100179108