List of publications

Huijbregts, L., Kjær Petersen, M.B., Berthault, C., Hansson, M., Aiello, V., Rachdi, L., Grapin-Botton, A., Honore, C., Scharfmann, R. (2019). Bromodomain and Extra Terminal Proteins Inhibitors Promote Pancreatic Endocrine Cell Fate. Diabetes, db180224. doi: 10.2337/db18-0224.

Dahl-Jensen, S.B., Yennek, S., Flasse, L., Larsen, H.L., Sever, G.K., Novak, I., Sneppen K. and Grapin-Botton, A. (2018). Deconstructing the principles of ductal network formation in the pancreas. PLoS Biology, 16, e2002842, doi:10.1371/journal.pbio.2002842.

Ramond, C., Beydag-Tasöz, B.S., Azad, A., van de Bunt, M., Petersen, M.K.B., Beer, , N.L., Glaser, N., Berthault, C., Gloyn, A.L., Hansson, M., McCarthy, M.I., Honoré, C., Grapin-Botton, A. and Scharfmann, R. (2018). Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling. Development 145, dev.165480, doi:10.1242/dev.165480.

Petersen, N., Frimurer, T.M., Pedersen, M.T., Egerod, K.L., Wewer Albrechtsen, N.J., Holst, J.J., Grapin-Botton, A., Jensen, K.B., Schwartz, T.W. (2018) Inhibiting RHOA Signaling in Mice Increases Glucose Tolerance and Numbers of Enteroendocrine and Other Secretory Cells in the Intestine. GastroenterologyS0016-5085, 34666-3. doi:10.1053/j.gastro.2018.06.039. 

Petersen, M.B.K., Gonçalves,C.A.C., Kim, Y.H. and Grapin-Botton, A. (2018) Recapitulating and Deciphering Human Pancreas Development From Human Pluripotent Stem Cells in a Dish. Current Topics in Developmental Biology 129, 143-190, doi:10.1016/bs.ctdb.2018.02.009.

Tsonkova, V.G., Sand, F.W., Wolf, X.A., Grunnet, L.G., Kirstine Ringgaard, A., Ingvorsen, C., Winkel, L., Kalisz, M., Dalgaard, K., Bruun, C., Fels, J.J., Helgstrand, C., Hastrup, S., Öberg, F.K., Vernet, E., Sandrini, M.P.B., Shaw, A.C., Jessen, C., Grønborg, M., Hald, J., Willenbrock, H., Madsen, D., Wernersson, R., Hansson, L., Jensen, J.N., Plesner, A., Alanentalo, T., Petersen, M.B.K., Grapin-Botton, A., Honoré, C., Ahnfelt-Rønne, J., Hecksher-Sørensen, J., Ravassard, P., Madsen, O.D., Rescan, C. and Frogne, T. (2018). The EndoC-βH1 cell line is a valid model of human beta cells and applicable for screenings to identify novel drug target candidates. Molecular Metabolism 8, 144-157, doi:10.1016/j.molmet.2017.12.007. 

Larsen, H.L., Martín-Coll, L., Nielsen, A.V., Wright, C.V.E., Trusina, A., Kim, Y.H. #Grapin-Botton, A. # (2017). Stochastic priming and spatial cues orchestrate heterogeneous clonal contribution to mouse pancreas organogenesis. Nature Communications 8, 605, doi:10.1038/s41467-017-00258-4. # Shared last authorship

Petersen, M.B.K., Azad, A., Ingvorsen, C., Hess, K., Hansson, M., Grapin-Botton, A.#, Honoré, C.# (2017). Single-Cell Gene Expression Analysis of a Human ESC Model of Pancreatic Endocrine Development Reveals Different Paths to β-Cell Differentiation. Stem Cell Reports 9:1246-1261. doi:10.1016/j.stemcr.2017.08.009. * Equal contribution. # Shared last authorship

Dahl-Jensen, S., Grapin-Botton, A. (2017). The physics of organoids: a biophysical approach to understanding organogenesis. Development 144, 946-951. doi:10.1242/dev.143693.

Martin, D., Grapin-Botton, A. (2017). The Importance of REST for Development and Function of Beta Cells. Frontiers in Cell and Developmental Biology 5,12, doi:10.3389/fcell.2017.00012.

Grapin-Botton, A., Serup, P. (2017). Parsing the Pancreas. New England Journal of Medicine 376, 886-888, doi:10.1056/NEJMcibr1616217.

Larsen, H.L., Grapin-Botton, A. (2017). The molecular and morphogenetic basis of pancreas organogenesis. Seminars in Cell and Developmental Biology 66, 51-68, doi:10.1016/j.semcdb.2017.01.005.

Berclaz, C., Szlag, D., Nguyen, D., Extermann, J., Bouwens, A., Marchand, P.J., Nilsson, J., Schmidt-Christensen, A., Holmberg, D., Grapin-Botton, A., Lasser, T. (2016).  Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow. Biomedical Optics Express 7, 4569-4580, doi: 1364/BOE.7.004569.

Grapin-Botton, A. (2016) Three-dimensional pancreas organogenesis models. Diabetes Obesity and Metabolism, Suppl 1, 33-40, doi:10.1111/dom.12720.

Dahl-Jensen, S.B., Figueiredo -Larsen, M., Grapin-Botton, A.#, Sneppen, K#. (2016). Short-range growth inhibitory signals from the epithelium can drive non-stereotypic branching in the pancreas. Physical Biology 13, 016007, doi:10.1088/1478-3975/13/1/016007. # Shared last authorship

Berclaz, C., Schmidt-Christensen, A., Szlag, D., Extermann, J., Hansen, L., Bouwens, A., Villiger, M., Goulley, J., Schuit, F., Grapin-Botton, A., Lasser, T., Holmberg, D. (2016). Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging. Diabetologia 59, 550-559, doi:10.1007/s00125-015-3819-x.

Martin, D., Kim, Y.H., Sever, D., Mao, C.A., Haefliger, J.A. and  Grapin-Botton. (2015). REST represses a subset of the pancreatic endocrine differentiation program.Developmental Biology 405, 316-327, doi: 10.1016/j.ydbio.2015.07.002. 

Ober, E.A. and  Grapin-Botton, (2015). At new heights - endodermal lineages in development and disease. Development 142, 1912-1917, doi: 10.1242/dev.121095.

Berclaz, C., Pache, C., Bouwens, A., Szlag, D., Lopez, A., Joosten, L., Ekim, S., Brom, M., Gotthardt, M., Grapin-Botton, A. and Lasser, T. (2015). Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers.Scientific Reports 5, 10385, doi: 1038/srep10385.

Grapin-Botton, A.,Seymour, P.A. and Gradwohl, G. (2015). Pairing-up SOX to kick-start beta cell genesis. Diabetologia 58, 859-61, doi: 10.1007/s00125-015-3539-2.

Kim, Y. H., List Larsen, H., Rué, P.,  Lemaire, L.A.,  Ferrer, J. and Grapin-Botton, A.  (2015). Cell cycle-dependent differentiation dynamics balances growth and endocrine differentiation in the pancreas. PLOS Biology 13:e1002111, doi:10.1371/journal.pbio.1002111.

Lemaire, L.A, Goulley, J., Kim, Y.H., Carat, S., Jacquemin, P.,   Rougemont, J., Constam, D.  and Grapin-Botton, A.(2015). Bicaudal C1 promotes pancreatic NEUROG3+ endocrine progenitor differentiation and ductal homeostasis. Development 142, 858-870, doi: 1242/dev.114611.

Rué, P., Kim, Y.H., List Larsen, H., Grapin-Botton, A., Martinez Arias, A. (2014). A framework for the analysis of symmetric and asymmetric divisions in developmental processes. BIORXIV/2014/010835, doi: 1101/010835.

Willet, S.G., Hale, M.A., Grapin-Botton, A.,Magnuson, M.A., MacDonald, R.J., Wright, C.V. (2014). Dominant and context-specific control of endodermal organ allocation by Ptf1a. Development 141, 4385-4394, doi: 1242/dev.114165.

Greggio, C., De Franceschi, F. and Grapin-Botton, (2014). Concise reviews: In Vitro-Produced Pancreas Organogenesis Models In Three Dimensions: Self-Organization From Few Stem Cells Or Progenitors. Stem Cells 33, 8-14, doi:10.1002/stem.1828.

Greggio, C., De Franceschi, F., Figueiredo-Larsen, M. and Grapin-Botton, (2014). In vitro pancreas organogenesis from dispersed mouse embryonic progenitors.Journal of Visual Expression 19. doi: 10.3791/51725, doi:10.3791/51725.

Hanotel, J., Bessodes, N., Thélie, A., Hedderich, M., Parain, K., Van Driessche, B., Brandão Kde, O., Kricha, S., Jorgensen, M.C., Grapin-Botton,, Serup, P., Van Lint, C., Perron, M., Pieler, T., Henningfeld, K.A. and Bellefroid, E.J. (2014). The Prdm13 histone methyltransferase encoding gene is a Ptf1a-Rbpj downstream target that suppresses glutamatergic and promotes GABAergic neuronal fate in the dorsal neural tube.Developmental Biology 386, 340-357, doi: 10.1016/j.ydbio.2013.12.024.

Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M. and Grapin-Botton, A. (2013). Artificial three-dimensional niches deconstruct pancreas development in vitro. Development 140, 4452-4462, doi: 1242/dev.096628.

Kartikasari, A.E., Zhou, J.X., Kanji, M.S., Chan, D.N., Sinha, A., Grapin-Botton, , Magnuson, M.A., Lowry, W.E. and Bhushan, A. (2013). The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation. EMBO Journal 32, 1393-1408, doi:10.1038/emboj.2013.78.

Qu, X., Afelik, S., Jensen, J.N., Bukys, M.A., Kobberup, S., Schmerr, M., Xiao, F., Nyeng, P., Veronica Albertoni, M., Grapin-Botton,  and Jensen, J. (2013). Notch-mediated post-translational control of Ngn3 protein stability regulates pancreatic patterning and cell fate commitment. Developmental Biology 376, 1-12, doi:10.1016/j.ydbio.2013.01.021.

Cortijo, C., Gouzi, M., Tissir, F. and Grapin-Botton, A. (2012). Planar cell polarity controls pancreatic beta cell differentiation and glucose homeostasis.Cell Reports 2, 1593-1606, doi:10.1016/j.celrep.2012.10.016.

Serup, P., Gustavsen, C., Klein, T., Potter, L.A., Lin, R., Mullapudi, N., Wandzioch, E., Hines, A., Davis, A., Bruun, C., Engberg, N., Petersen, D.R., Peterslund, J.M., Macdonald, R.J., Grapin-Botton,, Magnuson, M.A. and Zaret, K.S. (2012). Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice.Disease Models and Mechanisms 5, 956-966, doi:10.1242/dmm.009696.

Choi, E., Kraus, M.R.C., Lemaire L.A., Yoshimoto, M., Vemula, S, Potter, L.A., Manduchi, E., Stoeckert, C.J., Grapin-Botton, A. and Magnuson, M.A. (2012). Dual lineage-specific expression of Sox17 during mouse embryogenesis.Stem Cells 30, 2297-30, doi: 1002/stem.1192.

Kraus, M.R. and Grapin-Botton, (2012). Patterning and shaping the endoderm in vivo and in culture.Current Opinions in Genetics and Development 22, 347-53, doi:10.1016/j.gde.2012.05.002.

Berclaz, C., Goulley, J., Villiger, M., Pache, C., Bouwens, A., Martin-Williams, E., Van de Ville, D., Davison, A.C., Grapin-Botton, A. and Lasser, T. (2012). Diabetes imaging-quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy.Biomedical Optics Express 3, 1365-80, doi:1364/BOE.3.001365.

Shih, H.P., Kopp, J.L., Sandhu, M., Dubois, C.L., Seymour, P.A., Grapin-Botton, and Sander, M. (2012). A Notch-dependent molecular circuitry initiates pancreatic endocrine and ductal cell differentiation.Development 139, 2488-2499, doi:10.1242/dev.078634.

Thompson, N., Gésina, E., Scheinert, P., Bucher, P. and Grapin-Botton, A. (2012). Mechanisms of pancreas progenitor maintenance by Ptf1a are revealed by RNA profiling and ChIP sequencing.Molecular Cell Biology 32, 1189-1199, doi: 1128/MCB.06318-11.

Boutant, M., Ramos, O.H., Tourrel-Cuzin, C., Movassat, J., Ilias, A., Vallois, D., Planchais, J., Pégorier, J.P., Schuit, F., Petit, P.X., Bossard, P., Maedler, K., Grapin-Botton, and Vasseur-Cognet, M. (2012). COUP-TFII Controls Mouse Pancreatic β-Cell Mass through GLP-1-β-Catenin Signaling Pathways.PLoS One 7:e30847, doi: 10.1371/journal.pone.0030847.

Kraus, M. R-C,Clauin, S., Pfister,Y., Di Maïo, M., Ulinski, T., Constam, D., Bellanné-Chantelot, C. and Grapin-Botton, A. (2011). Two mutations in human BICC1 resulting in Wnt pathway hyperactivity associate with cystic renal dysplasia. Human Mutation 33, 86-90, doi:10.1002/humu.21610.

Gouzi, M., Kim, Y.H., Katsumoto, K., Johansson, K. and Grapin-Botton, A. (2011). Neurogenin3 initiates stepwise delamination of differentiating endocrine cells during pancreas development. Developmental Dynamics240, 589-604, doi: 1002/dvdy.22544.

Villiger, M., Goulley, J., Martin-Williams, E.J., Grapin-Botton, A. and Lasser, T. (2010). Towards high resolution optical imaging of beta cells in vivo. Current Pharmaceutical Design 16, 1595-608, doi:10.2174/138161210791164153.

Bayha, E., Jørgensen, M.C., Serup, P. and Grapin-Botton, A.(2009). Retinoic acid signaling organizes endodermal organ specification along the entire antero-posterior axis. PLoS One 4:e5845, doi:10.1371/journal.pone.0005845.

Villiger, M., Goulley, J., Friedrich, M.,Grapin-Botton, A., Meda, P., Lasser, T. and Leitgeb, R.A. (2009). In vivo imaging of endocrine islets with extended focus Optical Coherence Microscopy. Diabetologia 52, 1599-607, doi:10.1007/s00125-009-1383-y.

Grapin-Botton, A.(2008). Endoderm specification. In: StemBook.

Johansson, K., Dursun, U., Jordan, N., Gu, G., Beermann, F., Gradwohl, G. and Grapin-Botton, A. (2007). Temporal control of Neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cells.Developmental Cell 12, 457-65.

Grapin-Botton, A., Heimberg, H. and Lemaigre, F. (2007). The genetic programme of pancreatic beta-cells: basic science for the development of beta-cell therapy. Workshop on programming pancreatic beta-cells.EMBO Reports 8, 322-326, doi:10.1038/sj.embor.7400944.

Grapin-Botton, and Constam, D. (2007). Evolution of the mechanisms and molecular control of endoderm formation.Mechanisms of Development 124, 253-278, doi:10.1016/j.mod.2007.01.001.

Dessimoz, J., Opoka, R., Kordich, J.J., Grapin-Botton, A.and Wells, J.M. (2006). FGF signaling is necessary for establishing gut tube domains along the anterior-posterior axis in vivo. Mechanisms of Development 123, 42-45, doi:10.1016/j.mod.2005.10.001.

Dessimoz, J. and Grapin-Botton, A.(2006). Pancreas development and cancer: Wnt/beta-catenin at issue. Cell Cycle 5, 7-10, doi: 4161/cc.5.1.2293.

Dessimoz, J., Bonnard, C., Huelsken, J. and Grapin-Botton, A.(2005). Pancreas-specific deletion of b-catenin reveals Wnt-dependent and Wnt-independent functions during development. Current Biology 15, 1677-83, doi: 1016/j.cub.2005.08.037.

Grapin-Botton, A.(2005). Antero-posterior patterning of the vertebrate digestive tract: 40 years after Nicole Le Douarin's PhD thesis. International Journal of Developmental Biology 49, 335-47, doi: 1387/ijdb.041946ag.

Grapin-Botton, A.(2005). Ductal cells of the pancreas. International Journal of Biochemistry and Cell Biology 37, 504-510, doi: 10.1016/j.biocel.2004.07.010.

Grapin-Botton, A. and Constam, D. (2004). Endoderm development.  Cold Spring Harbor Laboratory Press. Editor: Claudio Stern.

Kumar, M., Jordan, N., Melton, D. and Grapin-Botton, A.(2003). Signals from lateral plate mesoderm instruct endoderm toward a pancreatic fate. Developmental Biology 259, 109-22, doi: 1016/S0012-1606(03)00183-0.

Johansson, K. and Grapin-Botton, A. (2002). Development and diseases of the pancreas. Clinical Genetics 62, 14-23, doi: 10.1034/j.1399-0004.2002.620102.x.

Grapin-Botton, A. (2002). Les étapes du développement du pancréas: des pistes pour le traitement du diabète. Médecine/Sciences 18, 467-473, doi: 10.1051/medsci/2002184467.

Ladjali-Mohammedi, K.,Grapin-Botton, A., Bonnin, M.A., Le Douarin, N.M. (2001). Distribution of HOX genes in the chicken genome reveals a new segment of conservation between human and chicken. Cytogenetic and Genome Research 92, 157-61, doi:10.1159/000056889.

Grapin-Botton, A., Majithia, A. R. and Melton, D.M. (2001). Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Genes & Development, 15, 444-454, doi: 10.1101/gad.846001.

Grapin-Botton,  and Melton, D.M. (2000). Endoderm development: from patterning to organogenesis.Trends in Genetics 16, 124-130, doi:10.1016/S0168-9525(99)01957-5.

Grapin-Botton, A., Cambronero, F., Weiner, H., Bonnin, M.-A., Puelles, L. and Le Douarin, N.M. (1999). Patterning signals acting in the spinal cord override the organizing activity of the isthmus.Mechanisms of Development 84, 41-53, doi: 10.1016/S0925-4773(99)00069-6.

Couly, G.,Grapin-Botton, A., Coltey, P., Ruhin, B. and Le Douarin, N.M. (1998). Determination of the identity of the derivatives of the cephalic neural crest : incompatibility between Hox-gene expression and lower jaw development. Development 125, 3445-59.

Nataf, V.,Grapin-Botton, A., Champeval, D., Amemiya, A.,Yanagisawa, A. and N.M. Le Douarin, N.M. (1998)The expression patterns of endothelin-A receptors and endothelin 1 in the avian embryo. Mechanisms of Development 75, 145-149. doi: 10.1016/S0925-4773(98)00079-3.

Mager, A-M., Grapin-Botton, A., Ladjali, K., Meyer, D., Wolff, C-M., Stiegler, P., Bonnin, M.-A., and Remy, P.(1998). The avian Fli gene is specifically expressed in a subset of neural crest cells giving rise to mesenchyme. International Journal of Developmental Biology 42, 561-72.

Grapin-Botton, A., Bonnin, M.-A. . Sieweke, M. and Le Douarin, N.M. (1998). Defined concentrations of a posteriorizing signal are critical for MafB/Kreisler segmental expression in the hindbrain. Development 125, 1173-1181.

Eichmann, A., Sieweke, M.,Grapin-Botton, A., Kelly, L., Graf, T. and Le Douarin, N.M. (1997). The expression pattern of MafB/Kr gene in birds and mice reveals that the kreisler phenotype does not represent a null mutant. Mechanisms of Development 65, 111-122, doi: 1016/S0925-4773(97)00063-4.

Le Douarin, N.M. and Grapin-Botton, A. (1997). Contrôle génétique du développement du rhombencéphale par les gènes Hox étudié chez l'embryon d'oiseau par la méthode des chimères caille-poulet. Comptes rendus des séances de la Société de biologie et de ses filiales 191, 29-42.

Grapin-Botton, A., Bonnin, M.-A. and Le Douarin, N.M. (1997). Hox gene induction in the neural tube depends on three parameters: competence, signal supply and paralogue group. Development124, 849-859.

Couly, G.,Grapin-Botton, A., Coltey, P. and Le Douarin, N.M. (1996). The regeneration of the cephalic neural crest, a problem revisited: the regenerating cells originate from the contralateral or from the anterior and posterior neural fold. Development 122, 3393-3407.

Le Douarin, N.M.,Grapin-Botton, A. and Catala, M. (1996). Patterning of the neural primordium in the avian embryo. Seminars in Cell and Developmental Biology 7, 157-167, doi: 1006/scdb.1996.0022.

Grapin-Botton, A., Bonnin, M.-A., McNaughton, L.A., Krumlauf, R. and Le Douarin, N.M.(1995). Plasticity of transposed rhombomeres: Hox gene induction is correlated with phenotypic modifications. Development 121, 2707-2721.