Serup Group: Developmental Biology of The Pancreas – University of Copenhagen

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Serup Group: Developmental Biology of The Pancreas

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Our aim is to understand the developmental biology of the pancreas. We wish to understand the signalling events that regulate growth and differentiation of pancreatic cell types with special emphasis on the insulin producing beta cell. 

We are particularly interested in understanding how Notch signalling regulates several distinct processes during pancreatic development. The majority of our studies are done with chicken, mice and embryonic stem cells. We use a wide variety of techniques including gene targeting, transgenic mice, gene arrays, immunohistochemistry, confocal microscopy, tissue explants, and in ovo electroporation. The aim of all our experiments is to understand the genetic and cellular interactions that direct pancreatic organogenesis.

Ongoing Projects:

We are currently mapping the temporal requirement of Notch signalling in different processes occurring during pancreatic development as well as determining the cell biological details of Notch signalling events. We are also involved in a collaborative tool-generating project that aims to develop new fluorescent reporters for a number of developmental signalling pathways. These tools will significantly improve our ability to study signalling events in vivo and in vitro.


Selected Publications:

Jorgensen MC, de Lichtenberg KH, Collin CA, Klinck R, Ekberg JH, Engelstoft MS, Lickert H & Serup P (2018). Neurog3-dependent Pancreas Dysgenesis Causes Ectopic Pancreas in Hes1 Mutants. Development, in press

Seymour, Philip A, Caitlin A Collin, Mette C Jorgensen, Itaru Imayoshi,  Ryoichiro Kageyama & Palle Serup (2018). Dll1 and Jag1 are differentially required to specify proximal and distal pancreatic duct compartments.bioRxiv 336529, doi: 10.1101/336529

de Lichtenberg, Kristian Honnens, Nina Sofi Funa, Nikolina Nakic, Jorge Ferrer, Zengrong Zhu, Danwei Huangfu & Palle Serup (2018). Genome- wide identification of HES1 target genes uncover novel roles for HES1 in pancreatic developmentbioRxiv 335869, doi: 10.1101/335869

Liu, Ka-Cheuk, Gunter Leuckx, Daisuke Sakano, Philip A. Seymour, Charlotte L. Mattsson, Linn Rautio, Willem Staels, Yannick Verdonck, Palle Serup, Shoen Kume, Harry Heimberg & Olov Andersson (2018). Inhibition of Cdk5 Promotes β-cell Differentiation from Ductal Progenitors. Diabetes, 67(1), 58-70, doi: 10.2337/db16-1587.

Stasiulewicz, Magdalena, Shona Gray, Ioanna Mastromina, Joana Clara Silva, Mia Bjorklund, Philip A. Seymour, David Booth, Calum Thompson, Rich Green, Emma A. Hall, Palle Serup & J. Kim Dale (2015). A conserved role for Notch in priming the cellular response to Shh through ciliary localisation of the key Shh transducer, Smoothened. Development, 142: 2291-303 doi:10.1242/dev.125237

Horn, Signe, Sune Kobberup, Mette C. Jørgensen, Mark Kalisz, Tino Klein, Ryoichiro Kageyama, Moritz Gegg, Heiko Lickert, Jill Lindner, Mark A. Magnuson, Young-Yun Kong, Palle Serup*, Jonas Ahnfelt-Rønne* & Jan N. Jensen (2012). Mind bomb 1 is required for pancreatic β-cell formation. PNAS 109(19), 7356-7361, doi: 10.1073/pnas.1203605109. *Corresponding author

Ahnfelt-Rønne, Jonas, Mette C. Jørgensen, Rasmus Klinck, Jan N. Jensen, Ernst-Martin Füchtbauer, Tye Deering, Raymond J. MacDonald, Chris V.E. Wright, Ole D. Madsen & Palle Serup (2012). Ptf1a-mediated control of Dll1 reveals an alternative to the lateral inhibition mechanism. Development, 139, 39-45, doi: 10.1242/dev.071761.

Kalisz, Mark, Maria Winzi, Hanne Catherine Bisgaard & Palle Serup (2012). EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression. Developmental Biology, 362(1), 94-103, doi:10.1016/j.ydbio.2011.11.017.

Serup, Palle, Carsten Gustavsen, Tino Klein, Leah A. Potter, Robert Lin, Nandita Mullapudi, Ewa Wandzioch, Angela Hines, Ashley Davis, Christine Bruun, Nina Engberg, Dorthe R. Petersen, Janny M. L. Peterslund, Raymond J. MacDonald, Anne Grapin-Botton, Mark A. Magnuson & Kenneth S. Zaret (2012). Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Disease Models & Mechanisms, 5(6), 956-966, doi:10.1242/dmm.009696.

Petersen, Dorthe R., Carsten Gustavsen, Søren R. Lindskog, Mark A. Magnuson, Kenneth S. Zaret & Palle Serup (2012). Engineering Artificial Signalling Centres to Polarize Embryoid Body Differentiation. Stem Cells and Development 21(4), 647 – 653, doi: 10.1089/scd.2011.0344